DNAdvance Performance and Data

Genomic DNA Isolation from Tissue Samples

The DNAdvance kit is a genomic DNA (gDNA) isolation reagent kit built on SPRI paramagnetic bead-based technology. It enables the purification of high quality DNA from tissue samples. The DNAdvance kit offers greater recovery of high quality gDNA than other commercially available DNA extraction kits. The extraction can be run manually in a 2 mL tube format or 96-well format, or automated in 96-well format on variety of Beckman Coulter Biomek liquid handling genomic workstations.

  • Compatible with PCR based downstream applications
  • Over 3 times the yield vs other suppliers’ extraction chemistries
  • Manual and automated workflows available

 

High Recovery of High-Quality gDNA

Genomic DNA was extracted from 20 mg liver tissue using DNAdvance and other suppliers’ kits. [Figure 1] Samples were quantified using the NanoDrop (Thermo Fisher Scientific). The DNAdvance kit recovered higher amounts of gDNA than the kits from the other suppliers. [Figure 2] Samples were accessed for purity using the NanoDrop (Thermo Fisher Scientific). Error bars represent the standard deviation of three technical replicates.

Genomics Genfind v2 DNA Yield

Figure 1

Genomics Genfind v2 DNA Purity

Figure 2

High Quality High Molecular Weight gDNA

 

Genomics DNAdvance from Tissue Types

Figure 3

DNAdvance isolates high quality gDNA. Genomic DNA isolated from 4 different tissue types using DNAdvance were run on the Agilent Genomic DNA Screen Tape to assess quality. DIN values from samples isolated using DNAdvance were of high quality for all tissue types. Sample electrograph traces of DNA extracted from tail (Figure 4) and kidney (Figure 5).

Genomics Genfind v2 DNA Yield

Figure 4

Genomics Genfind v2 DNA Purity

Figure 5

No Carryover Contaminants to Inhibit PCR Amplification or Downstream Applications

Genomics DNAdvance Tissue Amplify DNA

Figure 6

Figure 6: Ability to amplify DNA was assessed via qPCR using a primer set (forward primer 5´-ggacttcgagcaagagatgg-3´ and reverse primer 5´-agcactgtgttggcgtacag-3´) designed to span Exon 4 and 5 of the beta (β)-actin gene (ActB) to produce 327 base pair amplicons. The gDNA isolated from 4 tissue types isolated using the DNAdvance kit was amplifiable indicating that the kit removed PCR inhibitors.

 

DNA Extractions from a Variety of Tissue Types

The gDNA yield from five different tissue types. The yields were quantified using the NanoDrop (Thermo Fisher Scientific). The purity of samples was accessed using the NanoDrop (Thermo Fisher Scientific); the A260/280 ratios are shown in the last two columns and are satisfactory for downstream applications.

Tissue Type gDNA Tield (µG) DNA Purity (A260/A280)
Liver 33.5 1.9
Brain 8.07 1.8
Kidney 16.1 1.8
Lung 7.28 1.8
Tail 5.38 1.8

 

Visual Workflow

Genomics DNAdvance Workflow 
  1. Lyse tissue in Lysis Buffer and Proteinase K
  2. Bind DNA to magnetic beads
  3. Separate magnetic beads from contaminants
  4. Wash the magnetic beads with 70% ethanol to remove contaminants
  5. Repeat the wash step 3 times
  6. Elute DNA from magnetic beads
  7. Transfer to new plate

 

Extract DNA from Samples in Less Time with Less Pipette Movements

Genomics Genfind v2 DNA Yield

Figure 7

Genomics Genfind v2 DNA Purity

Figure 8

Automation Methods vs. Manual Timing

Estimated hands-on-time and total time in hours required to perform DNAdvance DNA extractions. The estimated time does not take into account the overnight lysis step. The chemistry can be performed either manually or automated on a Biomek Workstation. The table represents automated times preformed on a Biomek i-Series Multichannel with a 1200 µl head. The difference in time between manual and automation is indicated (NR=not recommended).

DNAdvance Manual Automated
Batch Size 8 Hands-on-Time 0.25 0.25
Total Time 0.50 0.87
24 Hands-on-Time 0.25 0.25
Total Time 0.50 0.87
96 Hands-on-Time NR 0.25
Total Time NR 0.87
480 Hands-on-Time NR 0.25
Total Time NR 2.25
960 Hands-on-Time NR 0.25
Total Time NR 4.25

 

DNAdvance Products for DNA Isolation from Tissue

DNAdvance reagent kit is available in two kit sizes based on your throughput needs. Click on the specific part number below for purchasing or request a quote. For more information check out our DNA isolation from tissue page featuring DNAdvance.

PART # NAME PREPS
A48705 DNAdvance Kit  384
A48706 DNAdvance Kit  9600

 

 

Not intended or validated for use in the diagnosis of disease or other conditions.
For Research Use Only. Not for use in diagnostic procedures.
All trademarks are property of their respective owners. Beckman Coulter, the stylized logo, Agencourt, AMPure, Biomek, GenFind, and SPRI are trademarks of Beckman Coulter, Inc. and are registered with the USPTO.

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